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Faculty of Engineering list:-

Attenuated Total Reflectance (ATR)/ Fourier Transform Infrared Spectroscopy
Centrifuge
CellTram Air
Electrophoresis System
Field Emission Transmission Electron Microscopy JEOL JEM 2010F
Gel Documentation System
KLA-Tencor P-15 Surface Profiler
Microplate Reader
Mercury Porosimeter
Plasma Cleaner
Planetary Ballmill
Perkin Elmer Pyris 6 Differential Scanning Calorimeter
Protein Purification System
Q800 Dynamic Mechanical Analyzer (DMA)
Surface Contact Angle - Visual Contact Angle (VCA) Optima
Thermal Cycler
TransferMan NK2
UV-VIS-NIR Spectrofluorometer
EPICS Altra Flow Cytometer

 

 

 

 

Field Emission Transmission Electron Microscopy JEOL JEM 2010F
The JEM-2010F Field Emission Electron Microscope is a multipurpose high resolution analytical electron microscope with high resolution image observation, microarea X-ray analysis, and with a wide range of capabilities.

Specifications:
Electron Gun Emitter: ZrO/W (100)
Accelerating Voltage: 100, 120, 160, 200 kV
Operation Modes: TEM, Lorenz, DIFF, NBED, CBED, STEM, EDS
Resolution: Point Image (0.19 nm) and Lattice Image (0.1nm)
Probe Size: 0.5nm
Magnification: TEM (x50~1,500,000) and STEM (x10,000~8,000,000)
Attachments: Slow Scan Charged-Coupled Diode (CCD) Camera GATAN 792, Fiber Optic Coupled TV Camera System GATAN 622 SC, Energy Dispersive X-Ray Spectrometers (EDS), OXFORD INCA, Scanning Transmission Electron Microscopy (STEM) JEOL

Applications:
Characterization of structure, crystallography, and elemental composition of a large variety material, for instance, ceramics, metals, semiconductors, polymers, and biological samples

Location:
NUSNNI FOE TEM Lab, E03-05-21
Contact:
Ms Xu Xiaojing (Tel: 68741623
)

 

CellTram Air

Features:
Optimized resolution for holding suspension cells
Work point can be set easily via pressure-compensation valve
Easy to use—oil filling is not required

Applications:
Gentle holding of suspension cells (e.g. oocytes)
Manual microinjection and dispensing of aqueous solutions
 

Location:
NUSNNI FOE Nano Biomechanics Lab, E03-05-16
Contact:
Mr Hairul (Tel: 68746564)

 

 

 

 

 

 

 

 

TransferMan NK2

TransferMan NK2 offers an excellent combination of automatic processes and intuitive operation with its improved proportional kinetics and menu-driven control. Even the most complex micromanipulation can be performed rapidly, simply, and precisely.

Features:
Improved proportional kinetics for intuitive control of manipulation
Menu-driven programming with storage of user profiles
Small steps for fine movement
High maximum speed for rapid work
Storage facility for three independent work positions
Integrated coarse and fine manipulator with a working range of >20 mm per axis
Programmable Z-axis limit for defined injection level avoids capillary breakage
Automated Home function for rapid capillary exchange
Can be adapted to all common microscopes

Applications:
> Micromanipulation of suspension cells
> ICSI
> PGD
> Pronuclear injection and ES transfer
> Nuclear transfer
> Microdissection
 

Location:
NUSNNI FOE Nano Biomechanics Lab, E03-05-16
Contact:
Mr Hairul (Tel: 68746564)

KLA-Tencor P-15 Surface Profiler

Specifications:
Stylus -based Scanning
Resolution up to 0.5 A
Scanning Area up to 200mm*200mm

Application:
P-15 is a highly sensitive surface profiler that measures step height, roughness and waviness on sample surfaces.

Location:
NUSNNI FOE Nanotechnology Facility Lab, E04-01-08
Contact:
Mr Wang Chen Chen (Tel: 68748309
)

Planetary Ballmill

Features:
Feed stock - soft, hard, brittle, fibrous
Feed size - <10mm
Final fineness - <1mm
Sample volume - max 4x250ml or 8x20ml


Applications:
Size reduction, mixing, homogenizing, mechanical alloying

Location:
NUSNNI FOE Materials Science Lab, E03-04-18
Contact:
Mr Abdul Khalim (Tel: 68742241)

 

UV-VIS-NIR Spectrofluorometer

Specifications:
Spectral range: 200-1400nm
Resolution < 0.5nm

Application:
The main purpose of the device is to capture fluorescence signal emitted from both solid and liquid samples

Location:
NUSNNI FOE Cellullar & Molecular Bioengineering Lab, E2-01-01
Contact:
Dr Zhang Yong (Tel: 68744871)

EPICS Altra Flow Cytometer

Specifications:
Four color analysis
Air cooled Argon , 15 mW, 488 nm operation
Cell sorting system (25000 events/sec)

Application:
For detection and identification of microorganisms and particle & cell sorting

Location:
NUSNNI FOE Confocal Microscopy & Flow Cytometry Lab, E3-05-14A
Contact:
Ms Xu ChengYu (Tel: 68743346)

 

 

Mercury Porosimeter

Specifications:
Pore range: 400- 0.0070 µm (70 Å)
2 Low pressure Station (able to do 2 runs at one time)
Low pressure range: 3.5-350 kPA (0.5-50 psi)
Low pressure range used when: Volumes of pores larger than 7 µm
High pressure range: 140 kPA-231 MPA (20-33000 psi)

Application:
To measure porosity of material via intrusion of mercury.

Location:
NUSNNI FOE ChBE lab, E4A-07-12
Contact:
Dr George Zhao (Tel: 6516-8081)

 

 

 

 

 

 

 

 

 

Q800 Dynamic Mechanical Analyzer (DMA)

Specifications:
Maximum force: 18N
Minimum force: 0.001N
Force Resolution: 0.0001N
Strain resolution: 1nm
Modulus range: 10E3 to 3x10E12 pa
Modulus Precision: +/- 1%
Tan delta range: 0.0001 to 10
Tan delta Sensitivity: 0.0001
Tan delta Resolution: 0.00001
Frequency range: 0.01 to 200 HZ
Dynamic Sample Deformation range: +/- 0.5 to 10,000 micrometer
Temperature range: -150 to 6000C
Heating rate: 0.1 to 200C/min
Cooling rate: 0.1 to 100C/min
Isothermal Stability: +/- 0.10C
Automated Furnace Movement: Yes
Time/Temperature Superposition Software: Yes

Applications:
DMA is a technique used to measure the mechanical properties of a wide range of materials. DMA differs from other mechanical testing devices in two ways. Firstly typical tensile test devices focus only on the elastic component. In many applications, the inelastic, or viscous component, is critical. It is the viscous component that determines properties such as impact resistance. Secondly, tensile test devices work primarily outside the linear viscoelastic range. DMA works primarily in the linear viscoelastic range and is therefore more sensitive to structure. The performance of a DMA is a function of its ability to apply force and measure displacement. The Q800 DMA incorporates the latest technology for this purpose. It offers all the major deformation modes required to characterize solid bars, elastomers, soft foams, thin films and fibers. The deformation modes include bending (single cantilever, dual cantilever and 3-point bend), shear, compression and tension. The Q800 uses air bearings for support of the drive shaft.

Location:
NUSNNI FOE Chemical & Physical Characterization Lab, E3-05-14B
Contact:
Ms Satinderpal Kaur (Tel: 68742162
)

 

 

Surface Contact Angle - Visual Contact Angle (VCA) Optima

Features:
Small and compact
Easy usage
The stage is made of aluminium with anodized hard coating
Ability to study both static and dynamic contact angle

Application:
Contact angle as a technique is sensitive to the chemical composition of the surface of a polymer material. It is a good way of studying the hydrophobicity or hydrophilicity of a material. It is a rather simple, inexpensive, and popular technique for characterizing polymer surface. However, one must take note that surface chemical composition can be inferred only indirectly from contact angle measurements and such interpretations are prone to artifacts. Together with other surface analysis techniques such as XPS, SIMS and ATRIR, conclusive results can be obtained. Two types of contact angle are available, static and dynamic contact angle. A static angle is formed at a stationary front while the dynamic angle is formed at a moving liquid front.
Location:
NUSNNI FOE Chemical & Physical Characterization Lab, E3-05-14B
Contact:
Ms Satinderpal Kaur (Tel: 68742162
)

 

 

 

 

 

 

 

 

 

 

Perkin Elmer Pyris 6 Differential Scanning Calorimeter

Features:
This is a heat Flux-DSC machine
Heat flow is determined by measuring a temperature difference over a very accurately known thermal resistance
Allows direct calorimetric measurement, characterization and analysis of thermal properties of materials
Study of endothermic and exothermic reactions
Able to perform isothermal experiments
Temperature range: -1200C-2000C and ambient-4500C
Heating rates: 0.1-500C/min (common range: 5-200C/min)
The furnace is designed for ruggedness and resistance to chemical corrosion. Because of its low mass, the furnace can heat and cool rapidly

A platinum resistance sensing device measures and controls the furnace temperature. This device, coupled with intrinsic “fuzzy logic” technology, provides very accurate furnace temperature control with minimal temperature overshoot
Sensor disk constructed of 90% nickel and 10% chromium
Highly sensitive thermocouples measure the temperature at both the sample and reference positions

Applications:
Sample preparation:
Most samples measured should be in the 5-30 mg range
Analyzes both solid and liquid samples. Solid samples can be in film, powder, crystal or granular form.
Proper sample preparation that maximizes the contact surface between the pan the sample will rescue any imbalance of the sample in the preformed sample pan and will result in maximum peak sharpness and resolution

Aluminium sample pans:
Used with nonvolatile solid samples such as plastics and polymers. Used in applications such as polymer melting, crystallization, glass transitions of polymers, thermoplastics and thermosets.

Location:
NUSNNI FOE Chemical & Physical Characterization Lab, E3-05-14B

Contact:
Ms Satinderpal Kaur (Tel: 68742162
)

 

 

 

 

 

 

 

 

 

 

Attenuated Total Reflectance (ATR)/ Fourier Transform Infrared Spectroscopy
To determine the type of functional groups present in a material. ATR enables the determination of functional groups at the surface of a material.

Features:
Standard pressure device tip is 2 mm diameter, which defines the effective spot size
Rugged Ge crystal has a spectral range of 4,000-675 cm-1
High refractive index of Ge yields shallow depth penetration – ideal for highly absorbing, carbon-filled samples and thin surface analyses
Dome shaped crystal design concentrates energy to a smaller spot size so smaller samples may be analyzed
Liquid holder allows small volumes of liquid to be sampled

Applications:
Single polymer beads
Single fibers
Carbon-filled materials such as O-rings
Liquids, including aqueous solutions, corrosive and caustics
Contaminants in paper

IR Microscope
Allows the study of extremely thin samples which cannot be studied with the Ge crystal (very common materials studied using the microscope accessory is the PCL film). It is also equipped with ATR *accessory
*This item isn't available at the moment. Yet to order

Manual Pellet Press Application:
Produce KBR pellets
Produce thin films
 

Location:
NUSNNI FOE Chemical & Physical Characterization Lab, E3-05-14B
Contact:
Ms Satinderpal Kaur (Tel: 68742162
)

 

 

 

 

 

 

 

 

 

 

Centrifuge

Features:
Less waiting with fast accel and decel rates
Four-liter batch throughput for bacteria and cell membrane isolation using the JLA-9.1000 J-Lite rotor at 16,800 x g
DNA sample preparation in up to 10 microplates with the JS-5.9 rotor
Live cell separation with the JE-6B elutriation system
Large volumes with low density of solid matter with the J-CFZ continuous flow system
Low heat output and low energy consumption
Maximum speed- 25,000rpm
Maximum force- 75,600rpm
Range-100-25,000rpm
Temperature range- -20’C to 40’C
Ambient range- 16’C to 38’C
Max set time- 9hrs 59mins and hold.

JLA_10.500 rotor:
M aximum speed – 10,000rpm
M aximum force – 18,500g
Maximum capacity – 6x 500ml

JA-25.50 Fixed angle rotor:
Maximum speed – 25,000rpm
Maximum force – 75,600g
Maximum capacity – 8x50 ml

Application:
Perform more separations than any other conventional high-speed centrifuges, while cutting run times
Offers faster acceleration, higher speeds and greater g-forces than conventional high-speed centrifuges (25,000 rpm and up to 75,000 x g - with larger volumes)

Location:
NUSNNI FOE Biochemistry Lab, E3-05-14
Contact:
Ms Satinderpal Kaur (Tel: 68742162
)

 

 

 

 

 

 

 

 

 

Plasma Cleaner

Features:
Compact, tabletop unit
Adjustable RF power
Low, Medium, and High power settings
Expanded model with a 6” diameter by 6” long chamber and an integral switch for a vacuum pump
Its hinged cover features a magnetic closure and a viewing window
Applies a maximum of 30W to the RF coil. No RF emission
Compliant with CE regulations
Size: 11"H x 18"W x 9"D.
Quartz plasma chamber is for lower possibility of contaminating the sample
Optional flow mixer allows individually metered intakes for up to two different gases and monitors the pressure in the chamber
Vacuum pump

Applications:
Cleaning optical, semiconductor, and other materials prior to coating or use in analytical equipment
Polymer surface modification
Pretreatment of biomedical and dental materials for sterilization, increased wettablity and bio-adhesion
Enhancing the adhesion of composite layers and improving wettability of channels for Lab-on-a-Chip and other micro-fluidic applications
Cleaning substrates prior to depositing Sol-Gel coatings
Improving glue adhesion for optical components, fiber optics, biomedical materials, aerospace and other applications
Cleaning slides, samples and specimen stages in fluorescence and electron microscopy
Pretreatment of silicone dental impression materials to increase wettability, producing more accurate gypsum replicas
Location:
NUSNNI FOE Biochemistry Lab, E3-05-14
Contact:
Ms Satinderpal Kaur (Tel: 68742162
)

 

 

 

 

 

 

 

 

 

 

 

Gel Documentation System

Features:
Document Images
Live image display for rapid acquisition
Software controlled integration to visualize faint samples
Saturated pixel identification for accurate analysis
Image contrast and pseudocolor adjustments for optimum image quality
Text annotation to label samples
Molecular weight determination
Concentration analysis

Benchtop Darkroom/Transilluminator Workstation
The Gel Doc 2000 system comes with its own darkroom and UV transilluminator workstation. Features include:
Closed system which eliminates UV light exposure to users
No need to turn off the laboratory lights or to operate in photographic darkrooms
Compact design which uses minimal laboratory space

High Sensitivity for Fluorescent Imaging
It is an easy-to-use, versatile system for the documentation and quantitation of a variety of fluorescent gel stains with excellent sensitivity. Features include:
• Real-time display or integration control (0.1–60 sec)
• Visualization of areas as small as 5 x 4 cm (8.5–51 mm motorized zoom lens)
• Visualization down to 0.1 ng of double-stranded DNA stained with ethidium bromide
New UV/White-Light Conversion Screen
The new UV/white-light conversion screen converts UV light to visible light, providing a convenient way to convert your UV transilluminator to a white-light source. Simply place the screen over a 302 nm UV transilluminator and document visible dyes such as Coomassie Blue and silver stain.

Applications:
Imaging and analyzing lane-based electrophoresis gels, 2D protein gel, dot blots, colonies, arrays and sequencing gels
Single-gel analysis but also creates and queries databases for sophisticated multiple-gel analysis
Quantitate and analyze a wide variety of biological data, including radioactive, chemiluminesceent, fluorescent and color-stained samples acquired from densitometers, phosphor images, fluorescent imagers and gel documentation systems

Location:
NUSNNI FOE Chemical & Physical Characterization Lab, E3-05-14B
Contact:
Ms Satinderpal Kaur (Tel: 68742162
)

Protein Purification System

Features:
Fast and easy protein purification
Reliability in every run
Flexibility for any purification
AKTA design support

Application:
Fast and easy protein purification

Location:
NUSNNI FOE Biochemistry Lab, E3-05-14
Contact:
Ms Satinderpal Kaur (Tel: 68742162
)

 

Electrophoresis System

Features:
Vertical mini-gel system for electrophoresis of small polyacrylamide or agarose gels
2 set glass plates
Rapid analysis of sample in short supply
Dual units enables the running of 2 mini-gels simultaneously under identical buffer conditions

Application:
Provides unparalleled resolution for applications ranging from purity assessments to analysis of complex protein mixtures
Apply full-size and mini-vertical slab cells and a horizontal isoelectric focusing cell

Location:
NUSNNI FOE Biochemistry Lab, E3-05-14
Contact:
Ms Satinderpal Kaur (Tel: 68742162
)

 

 

 

 

 

 

Microplate Reader

Features:
Can be configurated with 8 excitation filters and up to 8 emission filters
Fluorescence intensity/polarization
Time resolved fluorescence
Luminescence (optional)
For fluorescence application format up to 1536 wells
For absorbance, luminence and fluorescence polarization up to 384 wells that fulfill the SBS specification for microplate
Linear and orbital shaking
Incubation temperature +8°C to 45°C
Shortest reading time- 15s for 96 wells plate
30s for 384 wells plate
57s for 11536 wells plates

Application:
Fluorescence polarization for monitoring Ribozyme reaction in real rime
Homogenous fluorescence assays for miniaturized uHTS: drug-RNA interactions
Use of fluorescence polarization in a high through put assay for the identification of small molecules inhibitors
Quantification of protein-protein interaction using fluorescence polarization
Measurement of specific protease activity utilizing fluorescence polarization
Location:
NUSNNI FOE Biochemistry Lab, E3-05-14
Contact:
Ms Satinderpal Kaur (Tel: 68742162
)

 

 

 

 

 

 

 

Thermal Cycler

Features:
Hot Bonnet™ heated lid incorporates a thumbwheel to adjust height and pressure. This feature assures tight sealing of reaction vessels - even microplates - during oil-free thermal cycling (extra-cost option)
Precise Peltier-effect heat pumps - robustly designed for repeated cycling - deliver the full temperature range of 0°-100°C electronically, in a compact instrument with a footprint that measures 24cm x 28cm x 27cm high
Slide Griddle™ adapters allow many models to cycle up to four glass slides with speed and precision. Hold up to four glass microscope slides for in situ reactions.
Speedy ramping rates of up to 1°C/sec in models that hold 0.5ml tubes; up to 1.4°/sec in models that hold 0.2ml tubes or V-well plates; up to 2.5°/sec in models with the silver-gold block

Unparalleled temperature homogeneity: ±0.4°C within 30 seconds of arrival @ 60°C for all standard models
Elephantine memory stores approximately 320 programs, and cycler comes with 14 preprogrammed protocols. Any sequence of temperatures can be cycled up to 10,000 times; ramping rates less than maximum may be specified
Extremely accurate incubations, with NIST-traceable calibration. Any temperature including below-ambient temperatures may be held indefinitely. This feature allows refrigeration after an unattended run
Power-failure protection will resume program after power interruptions of up to twelve hours
Six standard models that can hold a wide variety of vessels, including 0.5ml & 0.2ml tubes, 96-well plates & glass slides

Application:
For amplified DNA
Reverse transcript

Location:
NUSNNI FOE Biochemistry Lab, E3-05-14
Contact:
Ms Satinderpal Kaur (Tel: 68742162
)

                                                                                                                                

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